One of the best ways to analyze your main character is to look at his or her morals. What is the one thing that they would never do and what situation would be that exception. If your character has a tendency to lie, especially to get things he/she wants, ask yourself in what situation would he/she stop lying and start telling the truth?
Some of the best stories are the ones where characters are thrown into situations where they wouldn’t usually find themselves. Place your stuck up corporate guy in a tattoo parlor or get your shy werewolf in a karaoke sing-off. Seriously, put characters in situations where they are uncomfortable and learn how he or she will react. The story doesn’t always have to follow these situations but these are great ways to get to know your characters and how she or he would react to strange situations.
Another great way to start building conflict is to know your characters basic needs like life, love, or money. These are all things that people, your readers, can relate to in most cultures. Take what your character needs away and surround it with fear of loss and conflict can begin to build.
Conflict also goes with the antagonist. Whatever your main character wants the antagonist wants the opposite. This doesn’t mean your antagonist is evil (well not to himself) it means that he has a perfectly good reason to want just the opposite. Think of this conflict as the game, both the main character and antagonist want to win but only one of them can have their way.
Creating conflict comes from opposition. Emotional conflict is just as important as physical conflict. In some cases emotional conflict, especially in books, is vital in developing character relationships and getting readers pulled into the story.
So create the most intense conflict you can think of and keep writing!